The function of Cas9 is to cleave the target DNA.
• A technology, which can be used to edit genes is CRISPR.
• It is a method of locating a specific bit of DNA within a cell, and then editing of the gene is generally done to change that piece of DNA.
• In CRISPR, the Cas9 protein is used to find and combine with almost any desired target sequence.
• The Cas9 protein combines with the guide RNA and then moves along the DNA strands until it locates and combines with the DNA sequence, which matches with the part of the guide RNA sequence.
• After that the standard Cas9 protein cleaves the DNA at the target, after repairing of the cut, the mutations are presented, which usually disable a gene.
Thus, the correct answer is option d, that is, cleave target DNA.
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