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After you performed the freeze-thaw cycle to lyse the bacteria, you pelleted the debris using a centrifuge. What two reasons did this pellet fluoresce green? (Yes, I know it contained GFP…. but why?)

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mshahzaib247

Answer:

Green Fluorescent Protein Labelled Bacteria

Explanation:

Green Fluorescent Protein labeling is very useful in studying prokaryotes. It is highly likely that the bacteria was also labelled. That's why after lysis and density level differentiation in a centrifuge the pellet start glowing green.

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