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Calculate the final concentration of ONPG (in mM) if you add 1.42 mL of 3.3 mM ONPG and dilute to a final volume of 10 mL with PBS buffer. Report your final answer to two places after the decimal.

Respuesta :

Answer : The final concentration of ONGP is, 0.47 mM

Explanation :

Formula used :

[tex]M_1V_1=M_2V_2[/tex]

where,

[tex]M_1\text{ and }V_1[/tex] are the initial molarity and volume

[tex]M_2\text{ and }V_2[/tex] are the final molarity and volume

We are given:

[tex]M_1=3.3mM\\V_1=1.42mL\\M_2=?\\V_2=10mL[/tex]

Now put all the given values in above equation, we get:

[tex]3.3mM\times 1.42mL=M_2\times 10mL\\\\M_2=0.47mM[/tex]

Hence, the final concentration of ONGP is, 0.47 mM

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