During the preparation of TSA plates, gelatin was accidently added to the media instead of agar. What would happen if these plates were used as streak plates for Micrococcus luteus and Micrococcus roseus and incubated at room temperature for 48 hours?

Question

03-11-2020
Biology

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During the preparation of TSA plates, gelatin was accidently added to the media instead of agar. What would happen if these plates were used as streak plates for Micrococcus luteus and Micrococcus roseus and incubated at room temperature for 48 hours?

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marianaegarciaperedo marianaegarciaperedo · Answer 1 · 2020-11-04T03:47:47+01:00

Answer and Explanation:

Nutritive gelatine was the first agent used to solidify a culture. With time, gelatin was replaced by agar, which has proven to be much better on many occasions. Gelatine has an animal origin (cartilage), while agar has a vegetable origin (algae).

The disadvantage of using gelatin is that it needs an incubation temperature of 20ºC or less to solidify. Many times this temperature is inferior to the optimal temperature needed by bacterias to grow. The optimal temperature of many Micrococcus species is about 25 and 30ºC. At these temperatures, gelatin would probably melt.

Another important aspect is that many organisms might consume the gelatine, metabolizing it. In nature, Micrococcus species might be found growing in products of animal origin.