An enzyme (MW 24 kDa, pI 5.5) is contaminated with two other proteins, one with a similar molecular mass and a pI of 7.0 while the other has a molecular mass of 100 kDa and a pI of 5.4. Suggest a procedure to purify the contaminated enzyme.

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03-08-2022
Chemistry

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An enzyme (MW 24 kDa, pI 5.5) is contaminated with two other proteins, one with a similar molecular mass and a pI of 7.0 while the other has a molecular mass of 100 kDa and a pI of 5.4. Suggest a procedure to purify the contaminated enzyme.

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luisvaschetto luisvaschetto · Answer 1 · 2022-08-04T17:46:43+02:00

The procedure to purify/separate these enzymes might be associated with the ion exchange chromatography methodology.

What is ion exchange chromatography methodology?

The ion exchange chromatography methodology is a technique to separate molecules such as proteins (in this case enzymes) based on their charge.

The ion exchange chromatography methodology is based on the use of different types of resins that allow the separation of molecules based on their charges and mass.

In conclusion, the procedure to purify/separate these enzymes might be associated with the ion exchange chromatography methodology.

Learn more about ion exchange chromatography here:

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