A Spectrophotometer measures enzyme activity by measuring the rate at which absorbance change. Absorbance means to measure the concentration of the product of an enzyme reaction.
How does low ph causes high absorbance?
Enzyme activity is at its maximum value at the optimum pH. As the pH value is increased above or decreased below the optimum pH the enzyme activity decreases.
So, when the medium of study is highly acidic the breaking of the bonds takes place easily. At lower pH values, the absorbance is high because the acid slows down the enzyme activity thereby changing its optimal pH and causes hydrolysis.
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