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The theme is a lesson about the human existence, a very grim lesson. Almost all characters admit to feeling lonely and isolated. Yet at their weakest point they still find ways to destroy those who are the weakest.
PHeck

Answer:

Loneliness.

Explanation:

The Predatory Nature of Human Existence. Of Mice and Men teaches a grim lesson about the nature of human existence. Nearly all of the characters, including George, Lennie, Candy, Crooks, and Curley's wife, admit, at one time or another, to having a profound sense of loneliness and isolation.

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