Out of the flowing given choices;
A) post-transcriptional processing.
B) electroporation.
C) post-translational processing.
D) nucleic acid hybridization.
E) restriction fragment ligation.
The answer is A. Bacteria do not have a complex post-transcriptional processing tool such as the one
in eukaryotes cells. Therefore, they are not able to process pre-mRNA with introns, example splicing. Therefore, a fully processed mRNA is cloned into
the bacterial cell so it is reverse-transcribed
into cDNA by reverse transcriptase.
